Selected publications

Chang PC, Yuan X, Zampieri A, Towns C, Yoo SP, Engstrom C, Tsai S, Robles CR, Zhu Y, Lopez S, Montel-Hagen A, Seet CS, Crooks GM. Generation of antigen-specific mature T cells from RAG1−/−RAG2−/−B2M−/− stem cells by engineering their microenvironment. Nature Biomedical Engineering. 2023 Dec 7. doi:10.1038/s41551-023-01146-7.

McAuley G, Yiu G, Chang PC, Newby GA, Campo-Fernandez B, Fitz-Gibbon ST, Wu X, Kang SL, Garibay A, Butler J, Christian V, Wong RL, Everette KA, Azzun A, Gelfer H, Seet CS, Narendran A, Murguia-Favela L, Romero Z, Wright N, Liu DR, Crooks GM, Kohn DB. Human T cell generation is restored in CD3δ severe combined immunodeficiency through adenine base editing. Cell. 2023 Mar 30. doi:10.1016/j.cell.2023.02.027.

Li S, Wang CS, Montel-Hagen A, Chen HC, Lopez S, Zhou O, Dai K, Tsai S, Satyadi W, Botero C, Wong C, Casero D, Crooks GM, Seet CS. Strength of CAR signaling determines T cell versus ILC differentiation from pluripotent stem cells. Cell Rep. 2023 Mar 28. doi:10.1016/j.celrep.2023.112241.

Montel-Hagen A, Tsai S, Seet CS, Crooks GM. Generation of Artificial Thymic Organoids from Human and Murine Hematopoietic Stem and Progenitor Cells. Curr Protoc. 2022 Apr. doi: 10.1002/cpz1.403

Sun, V., Sharpley, M., Kaczor-Urbanowicz, K. E., Chang, P., Montel-Hagen, A., Lopez, S., Zampieri, A., Zhu, Y., de Barros, S. C., Parekh, C., Casero, D., Banerjee, U., & Crooks, G. M. The Metabolic Landscape of Thymic T Cell Development in Vivo and In Vitro. Front. Immunol. 2021 July 28. doi.org/10.3389/fimmu.2021.716661

Montel-Hagen A, Sun V, Casero D, Tsai S, Zampieri A, Jackson N, Li S, Lopez S, Zhu Y, Chick B, He C, de Barros SC, Seet CS, Crooks GM. In Vitro Recapitulation of Murine Thymopoiesis from Single Hematopoietic Stem Cells. Cell Rep. 2020 Oct 27;33(4):108320. doi: 10.1016/j.celrep.2020.108320.

de Barros SC, Suterwala BT, He C, Ge S, Chick B, Blumberg GK, Kim K, Klein S, Zhu Y, Wang X, Casero D, Crooks GM. Pleiotropic Roles of VEGF in the Microenvironment of the Developing Thymus. J Immunol. 2020 Nov 1;205(9):2423-2436. doi: 10.4049/jimmunol.1901519. Epub 2020 Sep 28.

Montel-Hagen A, Seet CS, Li S, Chick B, Zhu Y, Chang P, Tsai S, Sun V, Lopez S, Chen HC, He C, Chin CJ, Casero D, Crooks GM. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019 Jan 4. pii: S1934-5909(18)30601-5. doi: 10.1016/j.stem.2018.12.011.

Montel-Hagen A, Crooks GM. From pluripotent stem cells to T cells. Exp Hematol. 2019 Mar;71:24-31. doi: 10.1016/j.exphem.2018.12.001. Epub 2018 Dec 24.

Calvanese V, Nguyen AT, Bolan TJ, Vavilina A, Su T, Lee LK, Wang Y, Lay FD, Magnusson M, Crooks GM, Kurdistani SK, Mikkola HKA. MLLT3 governs human haematopoietic stem-cell self-renewal and engraftment. Nature. 2019 Dec;576(7786):281-286. doi: 10.1038/s41586-019-1790-2. Epub 2019 Nov 27.

Chin CJ, Li S, Corselli M, Casero D, Zhu Y, He CB, Hardy R, Péault B, Crooks GM. Transcriptionally and Functionally Distinct Mesenchymal Subpopulations Are Generated from Human Pluripotent Stem Cells. Stem Cell Reports. 2018 Jan 3. pii: S2213-6711(17)30553-2. doi: 10.1016/j.stemcr.2017.12.005.

Seet CS, He C, Bethune MT, Li S, Chick B, Gschweng EH, Zhu Y, Kim K, Kohn DB, Baltimore D, Crooks GM* & Montel-Hagen A*. Generation of mature T cells from human hematopoietic stem and progenitor cells in artificial thymic organoids. Nature Methods 14, 521–530 (2017) doi:10.1038/nmeth.4237

Chin CJ, Cooper AR, Lill GR, Evseenko D, Zhu Y, He CB, Casero D, Pellegrini M, Kohn DB, Crooks GM. Genetic Tagging In Human Mesoderm Revealed Tripotent Lateral Plate Mesodermal Progenitors. Stem Cells. 34(5):1239-50. 2016. PMCID: PMC5052131

Casero D, Sandoval S, Seet CS, Scholes J,   Zhu Y, Ha VL, Luong A, Parekh C & Crooks GM. Long non-coding RNA profiling of human lymphoid progenitor cells reveals transcriptional divergence of B cell and T cell lineages. Nature Immunology. 2015 Dec; 16 (12):1282-91. PMCID PMC4653072

Kim WS, Zhu Y, Deng Q, Chin J, Grieco A, Dravid GG, Parekh C, Hollis RP, Lane T, Bouhassira E, Kohn DB, Crooks GM. Erythropoiesis from Human Embryonic Stem Cells Through Erythropoietin-Independent  AKT Signaling. Stem Cells. 32(6):1503-14, 2014. PMCID:PMC4037366

Chung B, Montel-Hagen A, Ge S, Zhu Y, Parekh C, Blumberg G, Arumugam B, Yang OO, Crooks GM. Engineering the Human Thymic Microenvironment to Support Thymopoiesis in Vivo, Stem Cells 32(9):2386-96, 2014. PMCID:PMC4138233.

Kohn LA, Seet CS, Scholes J, Codrea F, Chan R, Zaidi-Merchant S, Zhu Y, De Oliveira S, Kapoor N, Shah A, Abdel-Azim H, Kohn DB, Crooks GM. Human lymphoid development in the absence of common gamma chain receptor signaling. J Immunol. 2014 Jun 1;192(11):5050-8. PMCID: PMC4052377

Prashad SL, Calvanese V, Yao CY, Kaiser J, Sasidharan R, Crooks GM, Magnusson M, Mikkola HKA. GPI-80 Defines Self-renewal Ability in Hematopoietic Stem Cells during Human Development. Cell Stem Cell. 2014 Jan 8;16(1): 80-7. PMCID: PMC4520393

Corselli M, Chin CJ, Parekh C, Sahaghian A, Wang W, Ge S, Evseenko D, Wang X, Montelatici E, Lazzari L, Crooks GM, Péault B. Perivascular support of human hematopoietic stem/progenitor cells. BLOOD. 2013 Apr 11;121(15):2891-901. PMCID: PMC3707421

Evseenko D, Latour B, Richardson W, Corselli M, Sahaghian A,Cardinal S, Zhu Y, Chan R, Dunn B, Crooks GM. Lysophosphatidic Acid Mediates Myeloid Differentiation within the Human Bone Marrow Microenvironment. PLoS ONE 2013. 8(5): e63718. doi:10.1371/journal.pone.0063718 PMCID:PMC3655943

Parekh C, Crooks GM. Critical Differences in Hematopoiesis and Lymphoid Development Between Humans and Mice. Journal of Clinical Immunology 2013 May;33(4):711-5. PMCID: PMC3633618

Kohn LA, Hao, Q-L, Sasidharan R, Parekh C, Ge S, Zhu Y, Mikkola HKA, Crooks GM. Lymphoid Priming in Human Bone Marrow Begins Prior to CD10 Expression with Up-Regulation of L-selectin, Nature Immunology 2012 Oct;13(10):963-71. PMCID: PMC3448017

Parekh C, Sahaghian A, Kim W, Scholes J, Ge S, Zhu Y, Asgharzadeh S, Hollis R, Kohn DB, Ji L, Malvar J, Wang X, Crooks GM. Novel Pathways to Erythropoiesis Induced by Dimerization of Intracellular cMpl in Human Myeloid-Erythroid Progenitors, Stem Cells 2012 April; 30 (4): 697-708. PMCID:PMC3973153

Cuddihy AR, Suterwala B, Ge S, Kohn LA, Jang J, Andrade J, Wang X, Crooks GM. Rapid Thymic Reconstitution Following Bone Marrow Transplantation in Neonatal Mice is VEGF-Dependent. Biol Blood Marrow Transplant, 2012 May;18(5):683-9 PMCID: PMC3334422

Andrade J, Ge S, Symbatyan G, Rosol MS, Arthur J. Olch AJ, Crooks GM. Effects of Sub-lethal Irradiation on Patterns of Engraftment after Murine Bone Marrow Transplantation. Biol Blood Marrow Transplant. 2011 May;17(5):608-19. PMCID: PMC3086732

Dravid G, Zhu Y, Scholes J, Evseenko D, Crooks GM. Dysregulated Gene Expression During Hematopoietic Differentiation from human Embryonic Stem Cells. Mol Ther. 2011 Apr;19(4):768-81. PMCID: PMC3070091

Evseenko D, Zhu Y, Schenke-Layland K, Kuo J, Latour B, Ge, S, Dravid G, Li X, MacLellan WR, Crooks GM. Mapping the First Stages of Mesoderm Commitment During Differentiation of Human Embryonic Stem Cells. Proc Natl Acad Sci USA. 2010 Aug 3;107(31):13742-7.  PMCID: PMC2922221

Cuddihy A, Ge S, Zhu Y, Jang J, Chidgey A, Thurston G, Boyd R, Crooks GM. VEGF-Mediated Cross-talk Within the Neonatal Murine Thymus. Blood, 2009 Mar. 19;113(12):2723-31. PMCID: PMC2661859.

Evseenko D, Schenke-Layland K, Dravid G, Zhu Y, Hao QL, Scholes J, Wang XC, MacLellan R, Crooks GM. Identification of the critical extracellular matrix proteins that promote human embryonic stem cell assembly. Stem Cells and Development, 2009. Jul.-Aug; 18 (6): 919-28. PMID: 19021502.

Abdel-Azim H, Zhu Y, Hollis R, Wang X, Ge S, Hao QL, Smbatyan G, Kohn DB, Rosol M, and Crooks GM. Expansion of multipotent and lymphoid-committed human progenitors through intracellular dimerization of Mpl. Blood 2008; 15:111(8):4064-74. PMID: 18174381. PMCID: PMC2288719.

Hao QL, George AA, Zhu Y, Barsky LW, Zielinska, E, Wang X, Price, M., Ge Shundi, Crooks GM. Human intrathymic lineage commitment is marked by differential CD7 expression: Identification of CD7neg lympho-myeloid thymic progenitors. Blood 2008;111: 1318-1326. PMCID: 2214748.